Journal: Cell Death & Disease

Article Title: Reciprocal positive regulation between Cx26 and PI3K/Akt pathway confers acquired gefitinib resistance in NSCLC cells via GJIC-independent induction of EMT

doi: 10.1038/cddis.2015.197

Figure Lengend Snippet: Increased Cx26 is positively correlated with gefitinib resistance in NSCLC cells. ( a ) Differential expression of Cx26, Cx31.1, Cx32, and Cx43 in different gefitinib-sensitive NSCLC cell lines was determined by RT-PCR. ( b and c ) High level of Cx26 in gefitinib-insensitive A549 and H1299 cells than that in gefitinib-sensitive HCC827 and PC9 cells was detected by RT-PCR and western blotting. GAPDH or β -actin was used as internal loading control

Article Snippet: Human NSCLC cell lines (HCC827, PC9, A549, and H1299) were originally obtained from the ATCC (Manassas, VA, USA).

Techniques: Quantitative Proteomics, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

Journal: Cell Death & Disease

Article Title: Reciprocal positive regulation between Cx26 and PI3K/Akt pathway confers acquired gefitinib resistance in NSCLC cells via GJIC-independent induction of EMT

doi: 10.1038/cddis.2015.197

Figure Lengend Snippet: Cx26 induces acquired gefitinib resistance in NSCLC cells via GJIC-independent manner. ( a ) Functional GJIC was detected by parachute assay and no detectable GJIC was found in HCC827 GR, PC9 GR, and their parental cells. Top: fluorescence images. Bottom: overlaid the corresponding phase-contrast images. Original magnification, × 200. ( b ) No enhancement of GJIC in these cells incubated with 10, 20, and 40 μ M of RA (a well-defined GJIC enhancer) for 4, 8, 12, 24, and 48 h, respectively. Top: fluorescence images. Bottom: overlaid the corresponding phase-contrast images. Original magnification, × 200. ( c and d ) Immunofluorescence staining of the cellular localization of Cx26 with or without RA treatment. All scare bars represent 50 μ m

Article Snippet: Human NSCLC cell lines (HCC827, PC9, A549, and H1299) were originally obtained from the ATCC (Manassas, VA, USA).

Techniques: Functional Assay, Fluorescence, Incubation, Immunofluorescence, Staining

Journal: Cell Death & Disease

Article Title: Reciprocal positive regulation between Cx26 and PI3K/Akt pathway confers acquired gefitinib resistance in NSCLC cells via GJIC-independent induction of EMT

doi: 10.1038/cddis.2015.197

Figure Lengend Snippet: Cx26 and PI3K/Akt pathway functionally interplay to promote EMT and gefitinib resistance in NSCLC cells. ( a and b ) Effect of LY294002 or Akt overexpression on Cx26 expression in HCC827, PC9, and their GR cells was determined by western blotting. ( c ) Effects of Akt overexpression alone or combined with Cx26 overexpression or Cx26 depletion on cell morphology changes in HCC827 and PC9 cells. Original magnification, × 400. ( d – f ) Effects of Akt overexpression alone or combined with Cx26 overexpression or Cx26 depletion on the expression of EMT markers (E-cadherin, vimentin, and slug), cell migration, and invasion, as well as cell sensitivity to gefitinib in HCC827 and PC9 cells, respectively. Error bars are mean±S.D. from four independent experiments, ** P <0.01 versus vector group. # P <0.05 and ## P <0.01 versus Akt-overexpressing group

Article Snippet: Human NSCLC cell lines (HCC827, PC9, A549, and H1299) were originally obtained from the ATCC (Manassas, VA, USA).

Techniques: Over Expression, Expressing, Western Blot, Migration, Plasmid Preparation

Journal: International Journal of General Medicine

Article Title: Identification of Prognostic Factors Related to Super Enhancer-Regulated ceRNA Network in Metastatic Lung Adenocarcinoma

doi: 10.2147/IJGM.S332317

Figure Lengend Snippet: Identification of super enhancers in LUAD cells. ( A ) Enhancers were ranked according to the H3K27ac signals in HSC4 and A549 cells based on GSE143653. ( B ) Overlapping analysis of SE-associated genes in HSC4 and A549 cells. ( C ) Functional distribution of the overlapping genes.

Article Snippet: One normal human bronchial epithelial cell line (BEAS-2B) and six LUAD cell lines (H460, HCC827, A549, H1299, PC9 and Calu3) were obtained from American Type Culture Collection (ATCC, VA, USA).

Techniques: Functional Assay

Journal: International Journal of General Medicine

Article Title: Identification of Prognostic Factors Related to Super Enhancer-Regulated ceRNA Network in Metastatic Lung Adenocarcinoma

doi: 10.2147/IJGM.S332317

Figure Lengend Snippet: Effect of AC074117.1 on proliferation of LUAD cells. ( A ) qRT-PCR was used to detect the relative expression of AC074117.1 in BEAS-2B, H460, HCC827, A549, H1299, PC9 and Calu3 cells. ( B ) qRT-PCR was used to measure the knockdown efficiency of AC074117.1 in A549 and H1299 cells. ( C and D ) MTT assay was performed to evaluate changes in cell proliferation following AC074117.1 silencing in A549 and H1299 cells. **P<0.01.

Article Snippet: One normal human bronchial epithelial cell line (BEAS-2B) and six LUAD cell lines (H460, HCC827, A549, H1299, PC9 and Calu3) were obtained from American Type Culture Collection (ATCC, VA, USA).

Techniques: Quantitative RT-PCR, Expressing, Knockdown, MTT Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: SOD1 Promotes Cell Proliferation and Metastasis in Non-small Cell Lung Cancer via an miR-409-3p/SOD1/SETDB1 Epigenetic Regulatory Feedforward Loop

doi: 10.3389/fcell.2020.00213

Figure Lengend Snippet: Superoxide dismutase 1 (SOD1) expression is elevated in non-small cell lung cancer (NSCLC) tissues and cell lines. (A,B) SOD1 was overexpressed in both ADC and SCC tumors relative to normal tissues, and its expression also differed according to TNM stage as recorded in TCGA database. (C) Pan-cancer analysis of SOD1 expression. (D,E) Representative qRT-PCR and western blot analysis of SOD1 expression in 18 paired NSCLC clinical tissue specimens. Histogram of pooled data from NSCLC (T, n = 18) and adjacent normal lung (N, n = 18) tissue samples. Data are expressed as means ± standard deviation (SD). ∗ P < 0.05. (F) Representative immunohistochemistry images of NSCLC (T) and adjacent normal lung (N) tissue samples. Significantly darker brown staining of SOD1 protein was detected in cancer tissues (a) than in adjacent normal tissues (b) . Histogram of pooled data from NSCLC (tumor, n = 122) and normal lung (normal, n = 22) tissue. The percentage of NSCLC tissues with high SOD1 expression was significantly greater than that of normal lung tissues; ∗ P < 0.05. (G) qRT-PCR and western blot analysis of SOD1 expression in NSCLC cell lines and a normal bronchial epithelial cell line. The histogramillustrates of overexpression of SOD1 mRNA in NSCLC tumor tissues relative to normal lung tissues; ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: Seven NSCLC cell lines [A549, PC-9, NCI-H1299, NCI-H460, NCI-H1650, NCI-H520 and human bronchial epithelial cells (16HBE)] were purchased from American Type Culture Collection (ATCC, Manassas, VA, United States).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Standard Deviation, Immunohistochemistry, Staining, Over Expression

Journal: Frontiers in Cell and Developmental Biology

Article Title: SOD1 Promotes Cell Proliferation and Metastasis in Non-small Cell Lung Cancer via an miR-409-3p/SOD1/SETDB1 Epigenetic Regulatory Feedforward Loop

doi: 10.3389/fcell.2020.00213

Figure Lengend Snippet: Regulation of NSCLC cell proliferation, invasion, metastasis, apoptosis, and the cell cycle by SOD1. (A,B) SOD1 mRNA and protein expression levels were analyzed following transfection of SOD1 siRNA or pLVX-puro-SOD1 into H460 and H1299 cells. Representative western blot results are shown. (C,D) Proliferation of H460 and H1299 cells analyzed by CCK-8 assay after inhibition or overexpression of SOD1. (E,F) Evaluation of H460 and H1299 cell migration and invasion by transwell assay after inhibited or overexpressed of SOD1. (G–J) Measurement of apoptosis and the cell cycle by flow cytometryin cells with upregulated or downregulated SOD1 expression. Experiments were conducted using triplicate samples and each experiment was conducted three times; ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: Seven NSCLC cell lines [A549, PC-9, NCI-H1299, NCI-H460, NCI-H1650, NCI-H520 and human bronchial epithelial cells (16HBE)] were purchased from American Type Culture Collection (ATCC, Manassas, VA, United States).

Techniques: Expressing, Transfection, Western Blot, CCK-8 Assay, Inhibition, Over Expression, Migration, Transwell Assay

Journal: RSC Advances

Article Title: Silencing TRIM37 inhibits the proliferation and migration of non-small cell lung cancer cells

doi: 10.1039/c8ra06391e

Figure Lengend Snippet: TRIM37 is highly expressed in human NSCLC cell lines. (A) The mRNA level of TRIM37 in three kinds of cell lines (A549, PC9 and NCI-H1993) was significantly higher than that in control cells (BEAS-2B). (B) The protein level of TRIM37 in three kinds of cell lines (A549, PC9 and NCI-H1993) was significantly higher than that in control cells (BEAS-2B). Data represents the mean of three independent experiments. * p < 0.05.

Article Snippet: Three NSCLC cell lines (A549, PC9 and NCI-H1993) and a normal human bronchial epithelial cell line (BEAS-2B) were provided from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Control

Journal: RSC Advances

Article Title: Silencing TRIM37 inhibits the proliferation and migration of non-small cell lung cancer cells

doi: 10.1039/c8ra06391e

Figure Lengend Snippet: Knockdown of TRIM37 inhibits NSCLC cell proliferation in vitro . A549 cells were infected with sh-TRIM37 or mock for 24 h. (A) The mRNA level was significantly lower in cells transfected with sh-TRIM37. (B) The protein level was significantly lower in cells transfected with sh-TRIM37 than the mock group. (C) Cell proliferation was measured by the MTT assay in A549 cells. Data represents the mean of three independent experiments. * p < 0.05.

Article Snippet: Three NSCLC cell lines (A549, PC9 and NCI-H1993) and a normal human bronchial epithelial cell line (BEAS-2B) were provided from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Knockdown, In Vitro, Infection, Transfection, MTT Assay

Journal: RSC Advances

Article Title: Silencing TRIM37 inhibits the proliferation and migration of non-small cell lung cancer cells

doi: 10.1039/c8ra06391e

Figure Lengend Snippet: Knockdown of TRIM37 inhibits NSCLC cell migration and invasion in vitro . A549 cells were infected with sh-TRIM37 or mock for 24 h. (A) Cell migration was detected using the Transwell migration assay. (B) Cell invasion was measured using the Matrigel invasion assay. Data represents the mean of three independent experiments. * p < 0.05.

Article Snippet: Three NSCLC cell lines (A549, PC9 and NCI-H1993) and a normal human bronchial epithelial cell line (BEAS-2B) were provided from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Knockdown, Migration, In Vitro, Infection, Transwell Migration Assay, Invasion Assay

Journal: RSC Advances

Article Title: Silencing TRIM37 inhibits the proliferation and migration of non-small cell lung cancer cells

doi: 10.1039/c8ra06391e

Figure Lengend Snippet: Knockdown of TRIM37 inhibits EMT phenotype in NSCLC cells. A549 cells were infected with sh-TRIM37 or mock for 24 h. (A) Western blot was used to evaluate the protein levels of E-cadherin, N-cadherin and vimentin in knockdown and mock groups. (B) Quantitative analysis of E-cadherin, N-cadherin and vimentin. Data represents the mean of three independent experiments. * p < 0.05.

Article Snippet: Three NSCLC cell lines (A549, PC9 and NCI-H1993) and a normal human bronchial epithelial cell line (BEAS-2B) were provided from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Knockdown, Infection, Western Blot

Journal: RSC Advances

Article Title: Silencing TRIM37 inhibits the proliferation and migration of non-small cell lung cancer cells

doi: 10.1039/c8ra06391e

Figure Lengend Snippet: Knockdown of TRIM37 inhibits the growth of NSCLC in vivo . A549 cells transfected with sh-TRIM37 or mock were injected into the flanks of nude mice. (A) After 4 weeks, mice were euthanized and the tumors were weighed. (B) Tumor size was measured every 7 days. * p < 0.05.

Article Snippet: Three NSCLC cell lines (A549, PC9 and NCI-H1993) and a normal human bronchial epithelial cell line (BEAS-2B) were provided from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Knockdown, In Vivo, Transfection, Injection

Journal: RSC Advances

Article Title: Silencing TRIM37 inhibits the proliferation and migration of non-small cell lung cancer cells

doi: 10.1039/c8ra06391e

Figure Lengend Snippet: Knockdown of TRIM37 inhibits the activation of the Wnt/beta-catenin pathway in NSCLC cells. A549 cells were infected with sh-TRIM37 or mock for 24 h. (A) Western blot analysis was used to evaluate the levels of β-catenin, cyclinD1 and c-myc. (B) Quantitative analysis of β-catenin, cyclinD1 and c-myc. Data represents the mean of three independent experiments. * p < 0.05.

Article Snippet: Three NSCLC cell lines (A549, PC9 and NCI-H1993) and a normal human bronchial epithelial cell line (BEAS-2B) were provided from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Knockdown, Activation Assay, Infection, Western Blot